Description of the calculations involved in the method or a description of the computer program, and an example of the calculations. 11.2.1.2 The Probit Method, the Spearman-Karber Method, and the Trimmed Spearman-Karber Method are designed to produce LC50 values and associated 95% confidence intervals. It should be noted that software used to.
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Article citationsTSK (Trimmed Spearman-Karber) Program (1991) Version 1.5. Ecological Monitoring Research Division, Environmental Monitoring Systems Laboratory, US Environmental Protection Agency, Cincinnati.has been cited by the following article:.TITLE:AUTHORS:,KEYWORDS:JOURNAL NAME:,March26,2014ABSTRACT: Chlorpyrifosis a commonly used pesticide of organophosphate group, which causestoxicological effects in non-targetorganisms especially fish and frogs. In the present study, the genotoxicity ofsublethal concentrations of chlorpyrifos was observed in the erythrocytes ofcommon Indus valley toad, Bufo stomaticus,using the Alkaline Single-Cell Gel Electrophoresis (Comet) assay. In the firststep, acute toxicity of chlorpyrifos was evaluated by exposing the tadpoles tohigh concentrations of the pesticide. The acute LC50 value ofchlorpyrifos, calculated by Trimmed Spearman-Karber (TSK) in static bioassay,was found to be 930.0 μg/L.On the basis of acute LC50 value, the tadpoles were exposed to threesublethal concentrations (155, 233 and 465 μg/L) of chlorpyrifos for 96 h.
Blood cells werecollected at every 24 hinterval and were subjected to the Alkaline Single-Cell Gel Electrophoresisassay. The observed DNA damage was concentration and time-dependent, and those levels of DNAdamage in between the tested concentrations and times were significantly different(p Bufo stomaticus.
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